Human transforming growth factor

[54] HUMAN TRANSFORMING GROWTH FACTOR

[75] Inventors: Rik M. A. Derynck, Burlingame;

David V. Goeddel, Hillsborough,
both of Calif.

[73] Assignee: Genentech, Inc., South San
Francisco, Calif.

[21] Appl. No.: 695,494

[22] Filed: May 14, 1985

Related U.S. Application Data

[63] Continuation-in-part of Ser. No. 581,743, Feb. 17, 1984, abandoned.

[51] Int. CI.* C12P 21/00; C12P 21/02;

C12N 15/00; C12N 1/00

[52] U.S. CI 435/68; 435/70

435/172.3; 435/255; 530/324; 530/350: 530/828; 935/13; 935/28; 935/29; 935/57: 935/47; 935/48

[58] Field of Search 435/68, 172.3, 317,

435/70, 255; 260/112 R, 112 B; 536/27; 935/13, 27, 48, 29, 47, 56, 63, 73, 78; 514/12, 2;

530/324, 350, 828

[56] References Cited

U.S. PATENT DOCUMENTS

4,338,397 7/1982 Gilbert et al 435/68

4,358,586 11/1982 Rubin 536/27

4,511,503 4/1985 Olsen et al 260/112 R

4,546,082 10/1985 Kurjan et al 435/172.3

OTHER PUBLICATIONS

Itakura et al., Science, vol. 198, pp. 1056-1063, 1977, "Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone Somatostatin". Derynck et al., Cell, vol. 38 (1), pp. 287-297, Aug. 1984, "Human Transformig Growth Factor-Alpha: Precursor Structure and Expression in E. coli". Derynck et al., Nature, vol. 316, pp. 701-705, 1985, "Human Transforming Growth Factor-beta Complementary DNA Sequence and Expression in Normal and Transformed Cells".

Derynck et al., Cancer Cells, vol. 3, (Growth Factors Transform.), pp. 79-86, "Human Transforming Growth Factor-Alpha: Precursor Sequence, Gene Structure and Heterologous Expression".

Tame et al. Proc. Natl. Acad. Sci., vol. 83 (21), pp. 8082-8086, 1986, "Efficient Synthesis of Human Type

Alpha Transforming Growth Factor: Its Physical and Biological Characterization."

Marguardt et al., Proc. Natl. Acad Sci., vol. 80, pp. 4684-4688, Aug. 1983, "Transforming Growth Factors Produced by Retrovirus Transformed Rodent Fibroblust and Human Melanoma Cells: Amino Acid Sequence Homology with Epidermal Growth Factor. Marguardt et al., J. Biol. Chem., vol. 257 (9), May 10, 1982, pp. 5220-5225, "Human Transforming Growth Factor".

Toduro et al., Proc. Natl. Acad. Sci., vol. 77 (9), pp. 5258-5262, Sep. 1980, "Transforming Growth Factors Produced by Certain Human Tumor Cells: Polypeptides that Interact with Epidermal Growth Factor Receptors."

Anzano et al., Proc. Natl. Acad. Sci.,' vol. 80, pp. 6264-6268, Oct. 1983, "Surcoma Growth Factor From Conditioned Medium of Virally Transformed Cells is Composed of both Type a and Type j3 Transforming Growth Factors."

Kemp et al., "P/N.A.S. USA" 78(7): 4520-4524, (Jul. 1981).

Taniguchi et al., "P.N.A.S. USA" 77(9): 5230-5233,
(Sep. 1980).

Maniatis, T., "Molecular Cloning, A Laboratory Man-
ual" Cold Springs Harbor Lab., Summary 433, (1982).
Roberts, T. M., "Promoters, Structure & Function"
Rodriguez et al., Ed. 452-461, (1982).
Ohsuye, K., et al., "Nucl. Acids Res." 11(5): 1283-1295,

(1983) .

Kadonaga, J., et al., "J. Biol. Chem." 259(4): 2149-2154,

(1984) .

Roberts, A., et al., "P.N.A.S. USA" 77(6): 3494-3498, (Jun. 1980).

Sporn, M., et al., "Molecular Actions and Targets for
Cancer Chemotherapeutic Agents" Sartorelli, A., et al.,
Ed. 542-554, (1981).

Primary Examiner—Thomas G. Wiseman
Assistant Examiner—Robin Lyn Teskin