United States Patent [i9] [ii] Patent Number: 4,820,631
Lacal et al. [45] Date of Patent: Apr. 11, 1989
[54] DELETION MUTANTS AND MONOCLONAL ANTIBODIES AGAINST RAS PROTEINS
[75] Inventors: Juan C. Lacal, Bethesda, Md.; Stuart A . Aaronson, Vienna, Va.
[73] Assignee: The United States of America as
represented by the Secretary of the Department of Health and Human Services, Washington, D.C.
[21] Appl. No.: 890,510
[22] Filed: Jul. 30, 1986
[51] Int. a* C12Q 1/68; G01N 33/53;
G01N 33/577; C12N 5/00
[52] U.S. a 435/6; 435/7;
435/172.2; 435/810; 436/548; 436/813;
436/808; 530/387
[58] Field of Search 435/172.1, 172.2, 6,
435/849, 7, 68, 240.27, 810; 436/548, 811, 63, 64, 813, 808; 530/350, 387; 935/110, 103
[56] References Cited
U.S. PATENT DOCUMENTS
4,535,058 8/1985 Weinberg et al 436/813
4,699,877 10/1987 Cline et al 435/6
OTHER PUBLICATIONS
Clark et al., Proc. Natl. Acad. Sci., USA, vol. 82, 1985, pp. 5280-5284.
Furth et al., Journal of Virology, vol. 43, No. 1, 1982, pp. 294-304.
Lacal et al., (1986) Cell 44:609-617.
Lacal et al., (1986) The EMBO Journal 5:679-687.
Lacal et al., (1986) Molecular and Cell. Biology
6:1002-1009.
Primary Examiner—Esther M. Kepplinger
Assistant Examiner—Fiorina B. Hoffer
Attorney, Agent, or Firm—Holman & Stern
[57] ABSTRACT
Specific deletion mutants of ras p21 gene and specific monoclonal antibodies which recognize specific regions of the ras p21 protein have been prepared. A kit for detecting the presence of specific ras p21 proteins and their levels in a body sample has been described.
7 Claims, 6 Drawing Sheets
