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US006716583B2
(12) United States Patent ao) Patent No.: us 6,716,583 B2
Gold et al. (45) Date of Patent: Apr. 6,2004
(54) METHODS OF PRODUCING NUCLEIC ACID LIGANDS
(75) Inventors: Larry Gold, Boulder, CO (US); Craig Tuerk, Morehead, KY (US)
(73) Assignee: Gilead Sciences, Inc., Foster City, CA (US)
( * ) Notice: Subject to any disclaimer, the term of this patent is extended or adjusted under 35 U.S.C. 154(b) by 48 days.
(21) Appl. No.: 10/040,497
(22) Filed: Jan. 7, 2002
(65) Prior Publication Data
US 2002/0172962 Al Nov. 21, 2002
Related U.S. Application Data
(63) Continuation ol application No. 09/165,616, filed on Oct. 2, 1998, now Pat. No. 6,344,318, which is a continuation ol application No. 08/748,697, filed on Nov. 13,1996, now Pat. No. 5,817,785, which is a continuation ol application No. 08/442,062, filed on May 16,1995, now Pat. No. 5,595,877, which is a division ol application No. 07/964,624, filed on Oct. 21, 1992, now Pat. No. 5,496,938, which is a continuation-in-part ol application No. 07/714,131, filed on Jun. 10, 1991, now Pat. No. 5,475,096, which is a continuation-inpart ol application No. 07/536,428, filed on Jun. 11, 1990, now abandoned.
(51) Int. CI.7 C12Q 1/68; C12P 19/34;
G01N 33/566; C07H 21/02; C07K 14/00
(52) U.S. CI 435/6; 435/91.2; 436/501;
536/23.1; 536/25.4; 530/300; 530/350
(58) Field of Search 435/6, 91.2; 436/501;
530/300, 350; 536/22.1, 23.1, 24.5
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Primary Examiner—Stephanie Zitomer
Assistant Examiner—Monika Steinberg
(74) Attorney, Agent, or Firm—Swanson & Bratschun,
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