Matrices with memories and uses thereof

United States Patent [w]

Nova et al.

US006100026A [ii] Patent Number: 6,100,026 [45] Date of Patent: Aug. 8,2000

[54] MATRICES WITH MEMORIES AND USES THEREOF

[75] Inventors: Michael P. Nova, Rancho Santa Fe;

Andrew E. Senyei, La Jolla; Hanan
Potash, La Jolla, all of Calif.

[73] Assignee: Irori, San Diego, Calif.

[21] Appl. No.: 08/633,410 [22] Filed: Jun. 10, 1996

Related U.S. Application Data

[63] Continuation-in-part of application No. PCT/US96/06145, Apr. 25, 1996, which is a continuation-in-part of application No. 08/639,813, Apr. 2, 1996, abandoned, which is a continuation-in-part of application No. 08/567,746, Dec. 5, 1995, which is a continuation-in-part of application No. 08/538,387, Oct. 3, 1995, which is a continuation-in-part of application No. 08/480,147, Jun. 7, 1995, application No. 08/484,486, Jun. 7, 1995, application No. 08/484,504, Jun. 7, 1995, Pat. No. 5,751,629, application No. 08/480,196, Jun. 7, 1995, and application No. 08/473,660, Jun. 7, 1995, each is a continuation-in-part of application No.08/428,662, Apr. 25, 1995, Pat. No. 5,741,462.

[51] Int. CI.7 C12Q 1/68; G01N 33/53;

G01N 15/06; C12M 1/00

[52] U.S. CI 435/6; 435/7.1; 435/7.2;

435/7.3; 435/7.4; 435/287.1; 435/287.2; 435/288.1; 435/288.3; 422/58; 422/68.1;

422/119

[58] Field of Search 435/6, 5, 91.1,

435/91.2, 7.1-7.9, 808, 69.3, 69.6, 69.7, 287.1, 287.2, 287.9, 288.1, 288.3, 288.4, 288.7; 395/927, 842, 404, 49; 430/270.11; 356/301; 128/653; 422/64, 63, 55, 57, 58, 59, 60, 68.1, 119; 436/44

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Combinations, called matrices with memories, of matrix materials that are encoded with an optically readable code are provided. The matrix materials are those that are used in as supports in solid phase chemical and biochemical syntheses, immunoassays and hybridization reactions. The matrix materials may additionally include fluophors or other luminescent moieties to produce luminescing matrices with memories. The memories include electronic and optical storage media and also include optical memories, such as bar codes and other machine-readable codes. By virtue of this combination, molecules and biological particles, such as phage and viral particles and cells, that are in proximity or in physical contact with the matrix combination can be labeled by programming the memory with identifying information and can be identified by retrieving the stored information. Combinations of matrix materials, memories, and linked molecules and biological materials are also provided. The combinations have a multiplicity of applications, including combinatorial chemistry, isolation and purification of target macromolecules, capture and detection of macromolecules for analytical purposes, selective removal of contaminants, enzymatic catalysis, cell sorting, drug delivery, chemical modification and other uses. Methods for tagging molecules, biological particles and matrix support materials, immunoassays, receptor binding assays, scintillation proximity assays, non-radioactive proximity assays, and other methods are also provided.

24 Claims, 20 Drawing Sheets

Microfiche Appendix Included
(1 Microfiche, 53 Pages)

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