United States Patent [19] [ii] Patent Number: 4,945,051
Kikuchi et al. [45] Date of Patent: Jul. 31,1990
[54] PRODUCTION OF HUMAN LYSOZYME
[75] Inventors: Masakazu Kikuchi, Toyono; Koji
Yoshimura, Osaka; Kazuo Nakahama,
Nagaokakyo, all of Japan
[73] Assignee: Takeda Chemical Industries, Ltd., Osaka, Japan
[21] Appl. No.: 65,860
[22] Filed: Jun. 24, 1987
[30] Foreign Application Priority Data
Jun. 30, 1986 [JP] Japan 61-151809
Aug. 20, 1986 [JP] Japan 61-192639
Feb. 23, 1987 [JP] Japan 62-37869
[51] Int. CI.' C12N 15/00; C12N 9/36;
C12N 1/16; C07H 15/12
[52] U.S. CI 435/172.3; 435/206;
435/255; 435/320; 435/942; 536/27; 935/14;
935/28; 935/37; 935/48
[58] Field of Search 435/172.3, 206, 252.3,
435/255; 536/27; 935/14, 48
[56] References Cited
FOREIGN PATENT DOCUMENTS
OTHER PUBLICATIONS
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Oberto, J. et al., Gene 40, 57-65 (1985).
Muraki, M., et al., Argric. Biol. Chem. 50(3), 713-723
(1986).
Ikehara et al., Chem. and Pharm. Bull. vol. 34, No. 5, 5/86, pp. 2202-2208.
Jung et al, PNAS (USA), vol. 77, No. 10, 10/80, pp. 5759-5783.
Jigami et al. GENE 43: 273-279 (1986).
Kendall et al. Nature 321: 706-708 (1986, Jun. 12).
Yoshimura et al. Biochemical and Biophysical Research
Communications 145: 712-718 (1987, Jun. 15).
Primary Examiner—Thomas Mays
Attorney, Agent, or Firm—David G. Conlin; Ronald I.
Eisenstein
[57] ABSTRACT
A DNA sequence wherein a DNA segment coding for a signal peptide of the formula:
M-R-S-F-L-L-L-A-L-C-F-L-P-L-A-A-L-G
is bound to the 5' end of a DNA segment coding for human lysozyme, a cell transformed with the above DNA sequence and a process for producing human lysozyme, which comprises cultivating the above cell accumulating human lysozyme in the culture and recovering the same are disclosed.
The above techniques make the mass production of human lysozyme useful as pharmaceuticals possible. The present signal peptide is superior to that of hen egg white lysozyme for secretive production of human lysozyme.
8 Claims, 4 Drawing Sheets
