Isolated spinach ribulose-1,5-bisphosphate carboxylase/oxygenase large ...

aatcgtgcatatctcccagtatttgaaactttttgcaccttgacctaaacagttggtcta
tgtagagacttatattcaattttccattcaaaacaccggtactctagtattcatgtcgat
ttgatgtactagttttatgattctttgaactttctacgcgtctggtaaagggtcatcgat

SF-6 „

ctctgcttttcaaacagcttcacatcaaacttggcacttcattgtcattatttgttattc

^ SRzZ

ctacacggggttggacttgggagcaaggacgaacttcatctatctaaagccaattcaatg

tcgtattatatgtctgttgaagtctccagagttgtacggtagtatcttgtgttgaaatga

agaatgttgagtagtatagatctgtttttaattgtggtttagaggtatgttaaatttgga

aattggtttcaacgctaaaacactcttatttgacctgttcagagaaataaatcaatatgt
agacaatgaagggtttcttttgctggtagtttcaaatttgcctacttcacagtgatctat

SF-7

aagactataaatcttgcag GTAGTCAGTTGCTGAACACAACAfTGGGTGTGAAGGAGTTA

SQLLNTT LGVKE L

SQLLNTTLGVKEL SFM3

GTAGCAAATGAGTTTGCAAAACTGG^GGAAGAAGTACTAGTTCCCCACAAGCAACTATTC
VANEFAKLEEEVLVPHKQL F
VANEFAKLEEEVLVPHKQL F

SF-8*

Ccttttgatgtaactcaagatgacttcttttgggcatttggaatgctgcgatcaagagca"1
P Fdvtqddffwafgmlrsra
P Fdvtqddffwafgmlrsra

TTCACTTGTCTTGAGGGCCAAAGTCTTGTTCTAATCCCCTTGGCCGATTTG g t a a t c a t c
FTCLEGQSLVLI PLADL
FTCLEGQSLVLI PLADL

FIG. 2C SR-4 SPINACH40 MATLFTLIPS - SNSTFLNPFKTTQHSKLHFATPSPTFKNPLSIRCFRPPETDTPPEIQKFWGW 62

SF-9 ►

ttttctctcgaatcgaacaatgaagtatatataagtcacttaagtttaatgtcaactgct 2251

AGAACTTGTATTTTCACTGAGGAATCCAACCCCAGTTAAGGCTGGTGACCAG gtagtgtt 2191 §
ELVFSLRNPTPVKAGDQ 288'^
ELVFSLRNPTPVKAGDQ 284"

!Z5

actatcatggtccaagatacttagaatcaataattcaacag GTTCTGATCCAATACGATT 2311 «

V L I Q Y D L 295

V L I Q Y D L 291

©

FIG. 2E

C3
In

SF-11 ^ &

AGAAGGTGTTGCAACAGATTGATGAGGAATTCAAAGAAAGAGAGATGGAATTGGGTGGTT 2911 R
KVLQQI DEEFKEREMELGGY 468
KVLQQI DEEFKEREMELGGY 464

^ SR-2*

ACGAATACTACCAAGAACGGAGGCTTAAGGATCTTGGATTGGCCGGGGCACAGGGAGAGA 2971
EYYQERRLKDLGLAGAQGEK 488
EYYQERRLKDLGLAGAQGEK 484

c
s

AACTACCCTGGATAGGAGAGGTCTAATTATTTATAGAACACTTTTCTACTTCGTTTTCTT 3031 *

L P W I G E V 495
L P W I G E V 491

SPINACH38 MATLFTLIPS - SNSTFLNPFKTTQHSKLHFATPSPTFKNPLSIRCFRPPETDTPPEIQKFWGW 62

PEA MATIFSGG - - - SVSPFLFHTNKGTSFTPKAPILHLKRSFSAKSVASVGTEPSLSPAVQTFWKW 60

TOBACCO MASVFSVHPLPSSSFLCPLKTTKSRTKHHQTFYTYQKTILINSLQLTELDPKIPQPVQTFWQW 63

SPINACH40 LSDKGIISPKCPVKPGIVPEGLGLVAQKDIGRNEWLEVPQKFWINPDTVAASEIGSVCNGLK 125

SPINACH38 LSDKGIISPKCPVKPGIVPEGLGLVAQKDISRNEWLEVPQKFWINPDTVAASEIGSVCNGLK 125

PEA LQEEGVITAKTPVKASWTEGLGLVALKDISRNDVILQVPKRLWINPDAVAASEIGRVCSELK 123

TOBACCO LCKEGWTTKTPVKPGIVPEGLGLVAKRDIAKGETVLQVPKRFWINPDAVAESEIGNVCSGLK 126

SPINACH40 PWVSVALFLMREKKLGNSSSWKPYIDILPDSTNSTIYWSEEELSELQGSQLLNTTLGVKELVA 188

SPINACH38 PVWSVALFLMREKKLGNSSSWKPYIDILPDSTNSTIYWSEEELSELQGSQLLNTTLGVKELVA 188

PEA PWLSVILFLIRER - SREDSVWKHYFGILPQETDSTIYWSEEELQELQGSQLLKTTVSVKEYVK 185

TOBACCO PWISVALFLLREK - WRDDSKWKYYMDVLPKSTDSTIYWSEEELSEIQGTQLLSTTMSVKDYVQ 188

FIG. 3A