WO1994001446A3 - Derivatized organic solid support for nucleic acid synthesis - Google Patents

Derivatized organic solid support for nucleic acid synthesis Download PDF

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Publication number
WO1994001446A3
WO1994001446A3 PCT/US1993/006214 US9306214W WO9401446A3 WO 1994001446 A3 WO1994001446 A3 WO 1994001446A3 US 9306214 W US9306214 W US 9306214W WO 9401446 A3 WO9401446 A3 WO 9401446A3
Authority
WO
WIPO (PCT)
Prior art keywords
linker
nucleoside
nucleic acid
solid support
polymer
Prior art date
Application number
PCT/US1993/006214
Other languages
French (fr)
Other versions
WO1994001446A2 (en
Inventor
Parameswara M Reddy
Maged A Michael
Original Assignee
Beckman Instruments Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Instruments Inc filed Critical Beckman Instruments Inc
Publication of WO1994001446A2 publication Critical patent/WO1994001446A2/en
Publication of WO1994001446A3 publication Critical patent/WO1994001446A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Abstract

A solid-phase nucleotide synthesis intermediate useful for chemical synthesis of oligodeoxyribonucleotides and oligoribonucleotides comprises: (1) a particulate support comprising a porous polymer whose backbone comprises optionally substituted acrylate or methacrylate moieties; (2) a nucleoside; and (3) a linker having a first and a second end, the first end being covalently attached to the particulate support and the second end being covalently attached to the nucleoside, the linker spacing the nucleoside at least 3 atoms away from the polymer. The linker can comprise at least one optionally substituted aliphatic diamine. Alternatively, the linker can comprise a polyethylene glycol moiety. Preferably, the porous polymer is a methacrylate-vinylidene polymer. The solid-phase support thus produced can be used for oligodeoxyribonucleotide synthesis by either the phosphite-triester or the phosphotriester processes.
PCT/US1993/006214 1992-07-09 1993-06-29 Derivatized organic solid support for nucleic acid synthesis WO1994001446A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US91022392A 1992-07-09 1992-07-09
US07/910,223 1992-07-09

Publications (2)

Publication Number Publication Date
WO1994001446A2 WO1994001446A2 (en) 1994-01-20
WO1994001446A3 true WO1994001446A3 (en) 1994-03-03

Family

ID=25428489

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1993/006214 WO1994001446A2 (en) 1992-07-09 1993-06-29 Derivatized organic solid support for nucleic acid synthesis

Country Status (1)

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WO (1) WO1994001446A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992007882A1 (en) * 1990-10-26 1992-05-14 Genta Incorporated Non-aromatic organic polymeric reagents for solid phase synthesis of oligomers
US5554501A (en) * 1992-10-29 1996-09-10 Beckman Instruments, Inc. Biopolymer synthesis using surface activated biaxially oriented polypropylene
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
EP1958955B1 (en) * 1996-02-09 2013-09-04 Cornell Research Foundation, Inc. Spatially addressable oligonucleotide arrays and method of making the same
EP0912761A4 (en) 1996-05-29 2004-06-09 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
WO2000020643A1 (en) 1998-10-05 2000-04-13 Mosaic Technologies Reverse displacement assay for detection of nucleic acid sequences
US6995259B1 (en) 1998-10-23 2006-02-07 Sirna Therapeutics, Inc. Method for the chemical synthesis of oligonucleotides
CA2405412A1 (en) 2000-04-14 2001-10-25 Cornell Research Foundation, Inc. Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction
US6768005B2 (en) 2000-12-20 2004-07-27 Avecia Limited Process
WO2002046205A2 (en) * 2000-12-05 2002-06-13 Avecia Limited Process for the preparation of phosphorothionate oligonucleotides
US7205399B1 (en) 2001-07-06 2007-04-17 Sirna Therapeutics, Inc. Methods and reagents for oligonucleotide synthesis
US7279261B2 (en) 2005-01-13 2007-10-09 Xerox Corporation Emulsion aggregation toner compositions

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1982003079A1 (en) * 1981-03-10 1982-09-16 Inc Bioresearch Rapid solid phase synthesis of oligonucleotides using phosphorus oxychloride activation
WO1985001051A1 (en) * 1983-09-02 1985-03-14 Molecular Biosystems, Inc. Oligonucleotide polymeric support system
US4659774A (en) * 1985-11-01 1987-04-21 American Hoechst Corporation Support for solid-phase oligonucleotide synthesis
WO1992007864A1 (en) * 1990-10-26 1992-05-14 Genta Incorporated Improved process for the synthesis of oligomers
WO1992007882A1 (en) * 1990-10-26 1992-05-14 Genta Incorporated Non-aromatic organic polymeric reagents for solid phase synthesis of oligomers

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1982003079A1 (en) * 1981-03-10 1982-09-16 Inc Bioresearch Rapid solid phase synthesis of oligonucleotides using phosphorus oxychloride activation
WO1985001051A1 (en) * 1983-09-02 1985-03-14 Molecular Biosystems, Inc. Oligonucleotide polymeric support system
US4659774A (en) * 1985-11-01 1987-04-21 American Hoechst Corporation Support for solid-phase oligonucleotide synthesis
WO1992007864A1 (en) * 1990-10-26 1992-05-14 Genta Incorporated Improved process for the synthesis of oligomers
WO1992007882A1 (en) * 1990-10-26 1992-05-14 Genta Incorporated Non-aromatic organic polymeric reagents for solid phase synthesis of oligomers

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KATZHENDLER J. ET AL: "THE EFFECT OF SPACER, LINKAGE AND SOLID SUPPORT ON THE SYNTHESIS OF OLIGONUCLEOTIDES", TETRAHEDRON, vol. 45, no. 9, 1989, OXFORD, GB, pages 2777 - 2792, XP026629874, DOI: doi:10.1016/S0040-4020(01)80108-3 *

Also Published As

Publication number Publication date
WO1994001446A2 (en) 1994-01-20

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